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p nf κb signaling pathway activator pma  (Selleck Chemicals)


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    Selleck Chemicals p nf κb signaling pathway activator pma
    Nicotine reduces the sensitivity of colon cancer cells to oxaliplatin through modulation of the HMOX1/NF-κB signaling pathway. a-b The CCK-8 assay demonstrated that 10 µM oxaliplatin treatment for 24 h significantly inhibited the growth of LOVO and SW480 cells, while combined 1 µM nicotine treatment notably enhanced cell viability. Overexpression of HMOX1 reversed the 1 µM nicotine-induced increase in cell viability, and the <t>100</t> <t>nM</t> <t>p-NF-κB</t> pathway activator <t>PMA</t> treatment for 24 h partially restored this effect. c-d , k-l The scratch assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell migration ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. e-h The colony formation assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced enhancement of cell proliferation in LOVO and SW480 cells after 24 h, with 100 nM PMA treatment for 24 h partially restoring this effect. i-j , m-n The invasion assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell invasion ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. All data are expressed as mean ± standard deviation (independent triplicates). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, no significant difference
    P Nf κb Signaling Pathway Activator Pma, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+nf+%CE%BAb+signaling+pathway+activator+pma/Phorbol+12-myristate+13-acetate/pmc12999757-57-7-15
    Average 96 stars, based on 150 article reviews
    p nf κb signaling pathway activator pma - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity"

    Article Title: Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity

    Journal: Apoptosis

    doi: 10.1007/s10495-026-02308-z

    Nicotine reduces the sensitivity of colon cancer cells to oxaliplatin through modulation of the HMOX1/NF-κB signaling pathway. a-b The CCK-8 assay demonstrated that 10 µM oxaliplatin treatment for 24 h significantly inhibited the growth of LOVO and SW480 cells, while combined 1 µM nicotine treatment notably enhanced cell viability. Overexpression of HMOX1 reversed the 1 µM nicotine-induced increase in cell viability, and the 100 nM p-NF-κB pathway activator PMA treatment for 24 h partially restored this effect. c-d , k-l The scratch assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell migration ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. e-h The colony formation assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced enhancement of cell proliferation in LOVO and SW480 cells after 24 h, with 100 nM PMA treatment for 24 h partially restoring this effect. i-j , m-n The invasion assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell invasion ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. All data are expressed as mean ± standard deviation (independent triplicates). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, no significant difference
    Figure Legend Snippet: Nicotine reduces the sensitivity of colon cancer cells to oxaliplatin through modulation of the HMOX1/NF-κB signaling pathway. a-b The CCK-8 assay demonstrated that 10 µM oxaliplatin treatment for 24 h significantly inhibited the growth of LOVO and SW480 cells, while combined 1 µM nicotine treatment notably enhanced cell viability. Overexpression of HMOX1 reversed the 1 µM nicotine-induced increase in cell viability, and the 100 nM p-NF-κB pathway activator PMA treatment for 24 h partially restored this effect. c-d , k-l The scratch assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell migration ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. e-h The colony formation assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced enhancement of cell proliferation in LOVO and SW480 cells after 24 h, with 100 nM PMA treatment for 24 h partially restoring this effect. i-j , m-n The invasion assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell invasion ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. All data are expressed as mean ± standard deviation (independent triplicates). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, no significant difference

    Techniques Used: CCK-8 Assay, Over Expression, Wound Healing Assay, Migration, Colony Assay, Invasion Assay, Standard Deviation

    Related Articles

    CCK-8 Assay:

    Article Title: Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity
    Article Snippet: Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.. Iron death inducer RSL3 (Selleck, USA) and p-NF-κB signaling pathway activator PMA (Phorbol 12-myristate 13-acetate, Selleck, USA) were dissolved in DMSO to prepare stock solutions, stored at -20 °C in a dark environment, and diluted to working concentrations before use for cell treatment according to the experimental design.. According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced transcriptional activity of target genes [ ].According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced..

    Over Expression:

    Article Title: Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity
    Article Snippet: Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.. Iron death inducer RSL3 (Selleck, USA) and p-NF-κB signaling pathway activator PMA (Phorbol 12-myristate 13-acetate, Selleck, USA) were dissolved in DMSO to prepare stock solutions, stored at -20 °C in a dark environment, and diluted to working concentrations before use for cell treatment according to the experimental design.. According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced transcriptional activity of target genes [ ].According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced..

    Wound Healing Assay:

    Article Title: Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity
    Article Snippet: Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.. Iron death inducer RSL3 (Selleck, USA) and p-NF-κB signaling pathway activator PMA (Phorbol 12-myristate 13-acetate, Selleck, USA) were dissolved in DMSO to prepare stock solutions, stored at -20 °C in a dark environment, and diluted to working concentrations before use for cell treatment according to the experimental design.. According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced transcriptional activity of target genes [ ].According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced..

    Migration:

    Article Title: Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity
    Article Snippet: Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.. Iron death inducer RSL3 (Selleck, USA) and p-NF-κB signaling pathway activator PMA (Phorbol 12-myristate 13-acetate, Selleck, USA) were dissolved in DMSO to prepare stock solutions, stored at -20 °C in a dark environment, and diluted to working concentrations before use for cell treatment according to the experimental design.. According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced transcriptional activity of target genes [ ].According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced..

    Colony Assay:

    Article Title: Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity
    Article Snippet: Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.. Iron death inducer RSL3 (Selleck, USA) and p-NF-κB signaling pathway activator PMA (Phorbol 12-myristate 13-acetate, Selleck, USA) were dissolved in DMSO to prepare stock solutions, stored at -20 °C in a dark environment, and diluted to working concentrations before use for cell treatment according to the experimental design.. According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced transcriptional activity of target genes [ ].According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced..

    Invasion Assay:

    Article Title: Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity
    Article Snippet: Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.. Iron death inducer RSL3 (Selleck, USA) and p-NF-κB signaling pathway activator PMA (Phorbol 12-myristate 13-acetate, Selleck, USA) were dissolved in DMSO to prepare stock solutions, stored at -20 °C in a dark environment, and diluted to working concentrations before use for cell treatment according to the experimental design.. According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced transcriptional activity of target genes [ ].According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced..

    Standard Deviation:

    Article Title: Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity
    Article Snippet: Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.Oxaliplatin (MedChemExpress, USA) was dissolved in sterile water for standby.. Iron death inducer RSL3 (Selleck, USA) and p-NF-κB signaling pathway activator PMA (Phorbol 12-myristate 13-acetate, Selleck, USA) were dissolved in DMSO to prepare stock solutions, stored at -20 °C in a dark environment, and diluted to working concentrations before use for cell treatment according to the experimental design.. According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced transcriptional activity of target genes [ ].According to the official product manual of Selleck and relevant literature reports, the NF-κB pathway agonist PMA was treated with 100 nM as the working concentration to effectively activate the NF-κB pathway signal under no significant cytotoxicity conditions, promoting its nuclear translocation and enhanced..



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    p nf κb signaling pathway activator pma  (Selleck Chemicals)
    96
    Selleck Chemicals p nf κb signaling pathway activator pma
    Nicotine reduces the sensitivity of colon cancer cells to oxaliplatin through modulation of the HMOX1/NF-κB signaling pathway. a-b The CCK-8 assay demonstrated that 10 µM oxaliplatin treatment for 24 h significantly inhibited the growth of LOVO and SW480 cells, while combined 1 µM nicotine treatment notably enhanced cell viability. Overexpression of HMOX1 reversed the 1 µM nicotine-induced increase in cell viability, and the <t>100</t> <t>nM</t> <t>p-NF-κB</t> pathway activator <t>PMA</t> treatment for 24 h partially restored this effect. c-d , k-l The scratch assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell migration ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. e-h The colony formation assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced enhancement of cell proliferation in LOVO and SW480 cells after 24 h, with 100 nM PMA treatment for 24 h partially restoring this effect. i-j , m-n The invasion assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell invasion ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. All data are expressed as mean ± standard deviation (independent triplicates). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, no significant difference
    P Nf κb Signaling Pathway Activator Pma, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+nf+%CE%BAb+signaling+pathway+activator+pma/Phorbol+12-myristate+13-acetate/pmc12999757-57-7-15
    Average 96 stars, based on 1 article reviews
    p nf κb signaling pathway activator pma - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Nicotine reduces the sensitivity of colon cancer cells to oxaliplatin through modulation of the HMOX1/NF-κB signaling pathway. a-b The CCK-8 assay demonstrated that 10 µM oxaliplatin treatment for 24 h significantly inhibited the growth of LOVO and SW480 cells, while combined 1 µM nicotine treatment notably enhanced cell viability. Overexpression of HMOX1 reversed the 1 µM nicotine-induced increase in cell viability, and the 100 nM p-NF-κB pathway activator PMA treatment for 24 h partially restored this effect. c-d , k-l The scratch assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell migration ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. e-h The colony formation assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced enhancement of cell proliferation in LOVO and SW480 cells after 24 h, with 100 nM PMA treatment for 24 h partially restoring this effect. i-j , m-n The invasion assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell invasion ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. All data are expressed as mean ± standard deviation (independent triplicates). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, no significant difference

    Journal: Apoptosis

    Article Title: Nicotine suppresses ferroptosis in colon cancer cells via HMOX1/NF-κB pathway to reduce oxaliplatin sensitivity

    doi: 10.1007/s10495-026-02308-z

    Figure Lengend Snippet: Nicotine reduces the sensitivity of colon cancer cells to oxaliplatin through modulation of the HMOX1/NF-κB signaling pathway. a-b The CCK-8 assay demonstrated that 10 µM oxaliplatin treatment for 24 h significantly inhibited the growth of LOVO and SW480 cells, while combined 1 µM nicotine treatment notably enhanced cell viability. Overexpression of HMOX1 reversed the 1 µM nicotine-induced increase in cell viability, and the 100 nM p-NF-κB pathway activator PMA treatment for 24 h partially restored this effect. c-d , k-l The scratch assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell migration ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. e-h The colony formation assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced enhancement of cell proliferation in LOVO and SW480 cells after 24 h, with 100 nM PMA treatment for 24 h partially restoring this effect. i-j , m-n The invasion assay and quantitative analysis showed that HMOX1 overexpression reversed the 1 µM nicotine-induced increase in cell invasion ability in LOVO and SW480 cells after 24 h of treatment, with 100 nM PMA treatment for 24 h partially restoring this effect. Scale bar = 20 μm. All data are expressed as mean ± standard deviation (independent triplicates). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, no significant difference

    Article Snippet: Iron death inducer RSL3 (Selleck, USA) and p-NF-κB signaling pathway activator PMA (Phorbol 12-myristate 13-acetate, Selleck, USA) were dissolved in DMSO to prepare stock solutions, stored at -20 °C in a dark environment, and diluted to working concentrations before use for cell treatment according to the experimental design.

    Techniques: CCK-8 Assay, Over Expression, Wound Healing Assay, Migration, Colony Assay, Invasion Assay, Standard Deviation